Obviously we have not tried the first organisation in the list but they are on the approved bike hire company list put out by Deutsche Bahn (German Railways): 

BIOTOPIA Fahrradstation, 

Heinrich von Stephan Straße 2, 68161 Mannheim, T: 0621 1223077. It is a social organisation offering training and work to the long term unemployed. The building abuts platform 1 of the Mannheim Hauptbahnhof (Railway Station). The workshop repairs bikes as well.

Radsport Altig, Uhlandstr. 12, 68167 Mannheim, open from 10.00, 
T: 0621 3361386, F: 0621 - 3361387, eamil:, Internet: 

Opening times: Summer, 01. April – 30. September, Mo – Fr   10:00 – 13:00
and 14:00 – 19:00, Sat: 10:00 – 15:00

Prices 2015: Road bike, mountain bike, city bike and trekking (touring) bike 12 Euros / day. 
Tandem: 20 Euros / day
e-Bike: 25 Euros / day
Bike trailer available.

The Altig brothers Willi and Rudi were well known professional racing cyclists in the 1950s and 60s. The elder brother Willi took over a bicycle shop on his retirement from the sport.

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copyright: Judith & Neil Forsyth, Konrad-Adenauer-Allee 51A, D 68519 Viernheim